Development and characterization of a mass cytometry panel for detecting the effect of acute doxorubicin exposure on murine cardiac nonmyocytes

Abstract

Here, we describe the first mass cytometry studies of murine cardiac nonmyocytes. The mass cytometry panel is compared with single-cell RNA sequencing data using a novel approach of joint clustering with a deep neural network to assist in mass cytometry data labeling. Cardiac nonmyocytes in homeostasis are characterized by mass cytometry to identify replicating cells and variability in percent cellular composition of four major cell populations: endothelial cells, fibroblasts, leukocytes, and pericytes. The single-cell acute nonmyocyte response to doxorubicin is studied at 24 h and 72 h time points after doxorubicin exposure given daily for 5 days at a dose of 4 mg/kg/day, representative of acute, critical toxicity.