Microlymphatic and tissue oxygen tension in the rat mesentery

Abstract

Oxygen phosphorescence quenching was used to measure tissue Po2of lymphatic vessels of 43.6 ± 23.1 μm (mean ± SD) diameter in tissue locations of the rat mesentery classified according to anatomic location. Lymph and adipose tissue Po2were 20.6 ± 9.1 and 34.1 ± 7.8 mmHg, respectively, with the difference being statistically significant. Rare microlymphatic vessels in connective tissue not surrounded by microvessels had a Po2of 0.8 ± 0.2 mmHg, whereas the surrounding tissue Po2was 3.0 ± 3.2 mmHg, with both values being significantly lower than those of adipose tissue. Lower of lymph fluid Po2relative to the surrounding tissue was also evident in paired measurements of Po2in the lymphatic vessels and perilymphatic adipose tissue, which was significantly lower than the Po2in paired adipose tissue. The Po2of the lymphatic fluid of the mesenteric microlymphatics is consistently lower than that of the surrounding adipose tissue by ∼11 mmHg; therefore, lymph fluid has the lowest Po2of this tissue. The disparity between lymph and tissue Po2is attributed to the microlymphatic vessel wall and lymphocyte oxygen consumption.