Remote arteriolar dilations in response to muscle contraction under capillaries

Abstract

In hamster cremaster muscle, it has been shown previously that contraction of skeletal muscle fibers underlying small groups of capillaries (modules) induces dilations that are proportional to metabolic rate in the two arteriolar generations upstream of the stimulated capillaries (Berg BR, Cohen KD, and Sarelius IH. Am J Physiol Heart Circ Physiol 272: H2693–H2700, 1997). These remote dilations were hypothesized to be transmitted via gap junctions and not perivascular nerves. In the present study, halothane (0.07%) blocked dilation in the module inflow arteriole, and dilation in the second arteriolar generation upstream, the branch arteriole, was blocked by both 600 mosM sucrose and halothane but not tetrodotoxin (2 μM). Dilations in both arterioles were not blocked by the gap junction uncoupler 18-β-glycyrrhetinic acid (40 μM), and 80 mM KCl did not block dilation of the module inflow arteriole. These data implicate a gap junctional-mediated pathway insensitive to 18-β-glycyrrhetinic acid in dilating the two arterioles upstream of the capillary module during “remote” muscle contraction. Dilation in the branch arteriole, but not the module inflow arteriole, was attenuated by 100 μM N ω-nitro-l-arginine. Thus selective contraction of muscle fibers underneath capillaries results in dilations in the upstream arterioles that have characteristics consistent with a signal that is transmitted along the vessel wall through gap junctions, i.e., a conducted vasodilation. The observed insensitivities to 18-β-glycyrrhetinic acid, to KCl, and to N ω-nitro-l-arginine suggest, however, that there are multiple signaling pathways by which remote dilations can be initiated in these microvessels.