Hypoxia-induced alterations in Ca2+mobilization in brain microvascular endothelial cells

Author:

Kimura Chiwaka1,Oike Masahiro1,Ito Yushi1

Affiliation:

1. Department of Pharmacology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan

Abstract

To investigate the possible cellular mechanisms of the ischemia-induced impairments of cerebral microcirculation, we investigated the effects of hypoxia/reoxygenation on the intracellular Ca2+ concentration ([Ca2+]i) in bovine brain microvascular endothelial cells (BBEC). In the cells kept in normal air, ATP elicited Ca2+ oscillations in a concentration-dependent manner. When the cells were exposed to hypoxia for 6 h and subsequent reoxygenation for 45 min, the basal level of [Ca2+]i was increased from 32.4 to 63.3 nM, and ATP did not induce Ca2+ oscillations. Hypoxia/reoxygenation also inhibited capacitative Ca2+entry (CCE), which was evoked by thapsigargin (Δ[Ca2+]i-CCE: control, 62.3 ± 3.1 nM; hypoxia/reoxygenation, 17.0 ± 1.8 nM). The impairments of Ca2+ oscillations and CCE, but not basal [Ca2+]i, were restored by superoxide dismutase and the inhibitors of mitochondrial electron transport, rotenone and thenoyltrifluoroacetone (TTFA). By using a superoxide anion (O2 )-sensitive luciferin derivative MCLA, we confirmed that the production of O2 was induced by hypoxia/reoxygenation and was prevented by rotenone and TTFA. These results indicate that hypoxia/reoxygenation generates O2 at mitochondria and impairs some Ca2+mobilizing properties in BBEC.

Publisher

American Physiological Society

Subject

Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology

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