Affiliation:
1. Departments of Anesthesia and Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania 19104
Abstract
This study determined whether nociceptin/orphanin FQ (NOC/oFQ) generates superoxide anion (O2 −) in a protein kinase C (PKC)-dependent manner and whether such production contributes to hypoxic-ischemic (H-I) impairment of N-methyl-d-aspartate (NMDA)-induced pial artery dilation in newborn pigs equipped with closed cranial windows. Superoxide dismutase (SOD)-inhibitable nitroblue tetrazolium (NBT) reduction was an index of O2 − generation. Under non-H-I conditions, topical NOC/oFQ (10−10 M, concentration present in cerebrospinal fluid after I or H-I) increased SOD-inhibitable NBT reduction from 1 ± 1 to 20 ± 3 pmol/mm2. PKC inhibitors staurosporine and chelerythrine (10−7 M) blunted NBT reduction (1 ± 1 to 7 ± 2 pmol/mm2 for chelerythrine), whereas the NOC/oFQ receptor antagonist [F/G]NOC/oFQ (1–13)-NH2(10−6 M) blocked NBT reduction. [F/G]NOC/oFQ(1–13)-NH2 and staurosporine also blunted the NBT reduction observed after I or H-I. NMDA (10−8, 10−6 M)-induced pial artery dilation was reversed to vasoconstriction after H-I. The NOC/oFQ antagonist staurosporine and free radical scavengers partially prevented this impaired dilation (sham: 9 ± 1 and 16 ± 1; H-I: −5 and −10 ± 1; H-I staurosporine pretreated: 3 ± 1 and 6 ± 1%). These data show that NOC/oFQ increased O2 − production in a PKC-dependent manner and contributed to this production after insult and that NOC/oFQ contributed to impaired NMDA-induced pial artery dilation after H-I, suggesting, therefore, that PKC-dependent O2 −generation by NOC/oFQ links NOC/oFQ release to impaired NMDA dilation after H-I.
Publisher
American Physiological Society
Subject
Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology
Cited by
18 articles.
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