Affiliation:
1. Department of Physiology, The University of Liverpool, Liverpool L69 3BX; and
2. Unit of Cardiac Physiology, Department of Medicine, University of Manchester, Manchester M13 9PT, United Kingdom
Abstract
The effects of changing extracellular (pHo) and intracellular pH (pHi) on force and the mechanisms involved in the guinea pig portal vein were investigated to better understand the control of tone in this vessel. When pHo was altered, the effects on force and calcium were the same irrespective of whether force had been produced spontaneously by high-K depolarization or by norepinephrine; alkalinization increased tone, and acidification reduced it. Because pHo changes also lead to changes in pHi, we determined whether the effects on force could be explained by these induced pHi changes. It was found, however, that only with spontaneous activity did intracellular alkalinization increase force. In depolarized preparations, force was decreased, and, with norepinephrine, force was initially decreased and then increased. Thus the effects of pHo cannot be explained solely by changes in pHi. The role of the sarcoplasmic reticulum (SR) and surface membrane Ca2+-ATPase on the mechanism were investigated and shown not to be involved. Therefore, it is concluded that both pHo and pHi can have powerful modulatory effects on portal vein tone, that these effects are not identical, and that they are likely to be due to effects of pH on ion channels rather than the SR or plasma membrane Ca2+-ATPase.
Publisher
American Physiological Society
Subject
Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology
Cited by
2 articles.
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