Effects of thyroid hormones on cardiac development in oculo

Abstract

Embryonic rat myocardium was grafted into the anterior eye chamber (in oculo) of adult host rats in a series of two experiments that studied the effects of thyroid hormones [triiodothyronine (T3) and thyroxine (T4)] on heart tissue developing without hemodynamic load. In each experiment, surgical sympathectomy of one eye chamber was used to define possible interactions between thyroid hormones and sympathetic innervation to the graft. In the first experiment, propylthiouracil (PTU, 20 mg/kg sc) greatly suppressed growth and beating rate of whole heart grafts, while excess T4 (0.1 mg/kg sc) transiently increased beating rate but not growth. In the second experiment, T3 (5 mg/21 days, slow-release pellet) failed to promote growth in ventricular grafts. However, the size of atrial grafts in surgically sympathectomized eye chambers was larger in T3-treated compared with control rats. T3 administration increased beating rate in ventricular grafts. In both experiments, thyroid hormone treatments were effective in increasing heart weight-to-body weight ratios in the host rats. The data suggest that exposure to normal levels of thyroid hormones may be necessary for optimal cardiac growth. The data also support the hypothesis that a hemodynamic load is required for thyroid hormone-induced cardiac growth.