Author:
Sucharov Carmen C.,Mariner Peter D.,Nunley Karin R.,Long Carlin,Leinwand Leslie,Bristow Michael R.
Abstract
β-Adrenergic signaling plays an important role in the natural history of dilated cardiomyopathies. Chronic activation of β-adrenergic receptors (β1-AR and β2-AR) during periods of cardiac stress ultimately harms the failing heart by mechanisms that include alterations in gene expression. Here, we show that stimulation of β-ARs with isoproterenol in neonate rat ventricular myocytes causes a “fetal” response in the relative activities of the human cardiac fetal and/or adult gene promoters that includes repression of the human and rat α-myosin heavy chain (α-MyHC) promoters with simultaneous activation of the human atrial natriuretic peptide (ANP) and rat β-MyHC promoters. We also show that the promoter changes correlate with changes in endogenous gene expression as measured by mRNA expression. Furthermore, we show that these changes are specifically mediated by the β1-AR, but not the β2-AR, and are independent of α1-AR stimulation. We also demonstrate that the fetal gene response is independent of cAMP and protein kinase A, whereas inhibition of Ca2+/calmodulin-dependent protein kinase (CaMK) pathway blocks isoproterenol-mediated fetal gene program induction. Finally, we show that induction of the fetal program is dependent on activation of the L-type Ca2+channel. We conclude that in neonatal rat cardiac myocytes, agonist-occupied β1-AR mobilizes Ca2+stores to activate fetal gene induction through cAMP independent pathways that involve CaMK.
Publisher
American Physiological Society
Subject
Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology
Cited by
83 articles.
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