Author:
Yaras Nazmi,Tuncay Erkan,Purali Nuhan,Sahinoglu Babur,Vassort Guy,Turan Belma
Abstract
The present study was designed to determine whether the properties of local Ca2+ release and its related regulatory mechanisms might provide insight into the role of sex differences in heart functions of control and streptozotocin-induced diabetic adult rats. Left ventricular developed pressure, the rates of pressure development and decay (±dP/d t), basal intracellular Ca2+ level ([Ca2+]i), and spatiotemporal parameters of [Ca2+]i transients were found to be similar in male and female control rats. However, spatiotemporal parameters of Ca2+ sparks in cardiomyocytes isolated from control females were significantly larger and slower than those in control males. Diabetes reduced left ventricular developed pressure to a lower extent in females than in males, and the diabetes-induced depressions in both +dP/d t and −dP/d t were less in females than in males. Diabetes elicited a smaller reduction in the amplitude of [Ca2+]i transients in females than in males, a smaller reduction in sarcoplasmic reticulum-Ca2+ load, and less increase in basal [Ca2+]i. Similarly, the elementary Ca2+ events and their control proteins were clearly different in both sexes, and these differences were more marked in diabetes. Diabetes-induced depression of the Ca2+ spark amplitude was significantly less in females than in matched males. Levels of cardiac ryanodine receptors (RyR2) and FK506-binding protein 12.6 in control females were significantly higher than those shown in control males. Diabetes induced less RyR2 phosphorylation and FK506-binding protein 12.6 unbinding in females. Moreover, total and free sulfhydryl groups were significantly less reduced, and PKC levels were less increased, in diabetic females than in diabetic males. The present data related to local Ca2+ release and its related proteins describe some of the mechanisms that may underlie sex-related differences accounting for females to have less frequent development of cardiac diseases.
Publisher
American Physiological Society
Subject
Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology
Reference33 articles.
1. Bedinghaus J, Leshan L, Diehr S. Coronary artery disease prevention: what's different for women? Am Fam Physician 63: 1393–1400, 2001.
2. Gender differences in the generation of superoxide anions in the rat aorta
3. Brown RA, Filipovich P, Walsh MF, Sowers JR. Influence of sex, diabetes and ethanol on intrinsic contractile performance of isolated rat myocardium. Basic Res Cardiol 9: 353–360, 1996.
4. INFLUENCE OF GENDER AND DIABETES ON VASCULAR AND MYOCARDIAL CONTRACTILE FUNCTION
5. Sex differences in myocardial contractility in the rat
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