Roles of hyperpolarization-activated current If in sinoatrial node pacemaking: insights from bifurcation analysis of mathematical models

Abstract

To elucidate the roles of hyperpolarization-activated current ( If) in sinoatrial node (SAN) pacemaking, we theoretically investigated 1) the effects of If on stability and bifurcation during hyperpolarization of SAN cells; 2) combined effects of If and the sustained inward current ( Ist) or Na+ channel current ( INa) on robustness of pacemaking against hyperpolarization; and 3) whether blocking If abolishes pacemaker activity under certain conditions. Bifurcation analyses were performed for mathematical models of rabbit SAN cells; equilibrium points (EPs), periodic orbits, and their stability were determined as functions of parameters. Unstable steady-state potential region determined with applications of constant bias currents shrunk as If density increased. In the central SAN cell, the critical acetylcholine concentration at which bifurcations, to yield a stable EP and quiescence, occur was increased by smaller If, but decreased by larger If. In contrast, the critical acetylcholine concentration and conductance of gap junctions between SAN and atrial cells at bifurcations progressively increased with enhancing If in the peripheral SAN cell. These effects of If were significantly attenuated by eliminating Ist or INa, or by accelerating their inactivation. Under hyperpolarized conditions, blocking If abolished SAN pacemaking via bifurcations. These results suggest that 1) If itself cannot destabilize EPs; 2) If improves SAN cell robustness against parasympathetic stimulation via preventing bifurcations in the presence of Ist or INa; 3) If dramatically enhances peripheral cell robustness against electrotonic loads of the atrium in combination with INa; and 4) pacemaker activity of hyperpolarized SAN cells could be abolished by blocking If.