Strategies for inducing and validating zinc deficiency and zinc repletion

Author:

Wenegieme Tara-Yesomi1ORCID,Elased Dalia1,McMichael Kelia E.1,Rockwood Jananie1,Hasrat Khanzada1,Ume Adaku C.1ORCID,Marshall Andrea G.2ORCID,Neikirk Kit2,Kirabo Annet3ORCID,Elased Khalid M.4ORCID,Hinton Antentor2ORCID,Williams Clintoria R.1ORCID

Affiliation:

1. Department of Neuroscience, Cell Biology and Physiology; Boonshoft School of Medicine and the College of Science and Mathematics, Wright State University, Dayton, Ohio, United States

2. Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States

3. Department of Medicine, Vanderbilt University, Nashville, Tennessee, United States

4. Department of Pharmacology and Toxicology, Wright State University, Dayton, Ohio, United States

Abstract

This methods paper details dietary approaches to alter zinc homeostasis in rodents and qualitative and quantitative methods to ensure the zinc status of experimental animals. The outlined accessible and cost-effective protocol will elevate scientific rigor, ensuring reproducibility in studies exploring the impact of zinc deficiency and repletion on the onset and progression of a multitude of health conditions and diseases.

Funder

American Society of Nephrology KidneyCure Transition to Independence

American Heart Association

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases

Publisher

American Physiological Society

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