Aging-induced mitochondrial dysfunction: two distinct populations of mitochondria versus a combined population-Reference-Cited by-同舟云学术

Aging-induced mitochondrial dysfunction: two distinct populations of mitochondria versus a combined population

Published:2024-02-01 Issue:2 Volume:326 Page:H385-H395
ISSN:0363-6135
Container-title:American Journal of Physiology-Heart and Circulatory Physiology
language:en
Short-container-title:American Journal of Physiology-Heart and Circulatory Physiology

Author:

Chen Qun¹^ORCID,Thompson Jeremy¹,Hu Ying¹,Lesnefsky Edward J.¹²³⁴

Affiliation:

1. Division of Cardiology, Department of Medicine, Virginia Commonwealth University, Richmond, Virginia, United States

2. Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, Virginia, United States

3. Department of Physiology and Biophysics, Virginia Commonwealth University, Richmond, Virginia, United States

4. Department of Veterans Affairs Medical Center, Richmond, Virginia, United States

Abstract

We developed two methods to isolate mitochondria from a single mouse heart. We compared mitochondrial function in young and aged mice using mitochondria isolated with different methods. Both methods can be successfully used to isolate cardiac mitochondria from single mouse hearts. Our results provide the flexibility to isolate mitochondria from a single mouse heart based on the purpose of the study.

Funder

Virginia Commonwealth University

Center for Integrated Healthcare, U.S. Department of Veterans Affairs

HHS | NIH | National Institute on Aging

Publisher

American Physiological Society

Subject

Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpheart.00363.2023

Reference46 articles.

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2. Mitochondrial Function, Biology, and Role in Disease

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4. Loss of cardioprotection with ageing

5. Mitochondrial Dysfunction and Myocardial Ischemia-Reperfusion: Implications for Novel Therapies