Investigating feedforward neural regulation of circulation from analysis of spontaneous arterial pressure and heart rate fluctuations in conscious rats

Abstract

It has been suggested in anesthetized animals that the occurrence of sequences of consecutive beats characterized by systolic arterial pressure (SAP) and RR or pulse interval (PI) changing in the opposite direction (SAP+/RR− and SAP−/RR+, nonbaroreflex sequences) might represent the expression of neural cardiovascular regulatory mechanisms operating with feedforward characteristics. The aim of the present study was to study nonbaroreflex sequences in a more physiological experimental model, i.e., in conscious freely moving rats. We studied conscious rats before and after 1) complete autonomic blockade ( n = 12), 2) sympathetic blockade ( n = 10), 3) α ( n = 7)- and β ( n = 8)-adrenergic blockade, and 4) parasympathetic blockade ( n = 10). Nonbaroreflex sequences were defined as three or more beats in which SAP and PI of the following beat changed in the opposite direction. Complete autonomic blockade reduced the number of nonbaroreflex sequences (95.6 ± 9.0 vs. 45.2 ± 4.1, P < 0.001), as did sympathetic blockade (80.9 ± 12.6 vs. 30.9 ± 6.1, P < 0.001). The selective α-receptor blockade did not induce significant changes (80.9 ± 12.5 in baseline vs. 79.0 ± 14.7 after prazosin), whereas β-receptor blockade significantly reduced nonbaroreflex sequence occurrence (80.9 ± 12.5 in baseline vs. 48.9 ± 15.3 after propranolol). Parasympathetic blockade produced a significant increase of nonbaroreflex sequences (95.1 ± 6.9 vs. 136.0 ± 12.4, P < 0.01). These results demonstrate the physiological role of the nonbaroreflex sequences as an expression of a feedforward type of short-term cardiovascular regulation able to interact dynamically with the feedback mechanisms of baroreflex origin in the neural control of the sinus node.