Temporal Coding of Intensity of NaCl and HCl in the Nucleus of the Solitary Tract of the Rat

Abstract

Sensory neurons are generally tuned to a subset of stimulus qualities within their sensory domain and manifest this tuning by the relative size of their responses to stimuli of equal intensity. However, response size alone cannot unambiguously signal stimulus quality, since response size also depends on stimulus intensity. Thus a common problem faced by sensory systems is that response size (e.g., spike count) confounds stimulus quality and intensity. Here, using the gustatory system as a model, we asked whether temporal firing characteristics could disambiguate these axes. To address this question, we recorded taste responses of single neurons in the nucleus of the solitary tract (NTS, the first central gustatory relay) in anesthetized rats to a range of concentrations of NaCl and HCl and their binary mixtures. To assess the contribution of the temporal characteristics of the response to discrimination among tastants, a family of metrics that quantifies the similarity of two spike trains in terms of spike count and spike timing was used. Results showed that the spike count produced by different taste qualities and different concentrations overlapped in most cells, implying that information conveyed by spike count is imprecise. Multidimensional scaling analysis of taste responses using similarity of temporal characteristics showed that different taste qualities, intensities, and mixtures formed distinct clusters in this “temporal coding” taste space and were arranged in a logical order. Thus the temporal structure of taste responses in single cells in the NTS can simultaneously convey information about both taste quality and intensity.