Diffuse reflectance spectroscopy for identification of carcinogen transformation stages in skin tissue

Abstract

Abstract Today, to establish a diagnosis, the patient must undergo a biopsy followed by histopathological diagnosis, which causes unnecessary cost, patient trauma, and time delay to obtain a diagnosis. However, the metastases can be discovered by diffuse reflectance spectroscopy, which is a simple method that investigates the light distribution within tissue. The theme of this paper is the use of diffuse reflectance spectroscopy (DRS) to determine the optical spectrum of hamster specimen’s tissue and to differentiate biological changes due to laser irradiation (scattering, and cell changes) under the skin. DRS measurements were made on healthy and malignant tissue to diagnose the stages of cancer formation using a fiber-optic probe. The results show that malignant tissue is characterized by a significant decrease in diffuse reflectance spectrum compared to normal tissue.