Volumetric Assessment of Airborne Indoor and Outdoor Fungi at Poultry and Cattle Houses in the Mazandaran Province, Iran

Abstract

Volumetric Assessment of Airborne Indoor and Outdoor Fungi at Poultry and Cattle Houses in the Mazandaran Province, IranThe aim of this study was to assess the volume of airborne fungi in the indoor and outdoor environment of poultry and cattle houses in the Mazandaran Province in Iran. Indoor and outdoor air of twenty cattle houses and twenty-five poultry houses were sampled using a single-stage impactor, which draws air at 20 L min-1and impacts sampled material onto Petri plates containing malt extract agar. The plates were incubated at 30 °C for seven days, after which the resulting colonies were counted. The fungi were identified and counted microscopically and macroscopically. A total of 4,662 fungal colonies were isolated from 90 plates collected from indoor and outdoor air of cattle and poultry houses.Cladosporium(55.3 %), yeast (10.0 %), andAspergillus(9.4 %) were the most common findings. The concentration of airborne fungi in cattle and poultry houses ranged from 10 CFU m-3to 1700 CFU m-3in indoor and 10 CFU m-3to 2170 CFU m-3in outdoor environments.Cladosporiumhad the highest mean indoor (424.5 CFU m-3) and outdoor (449.7 CFU m-3) air concentration in the cattle houses. In the poultry houses, the highest mean concentrations were measured forCladosporium(551.0 CFU m-3) outdoors and yeast (440.7 CFU m-3) indoors. These levels might present an occupational risk, but threshold levels for these environments have yet to be established worldwide.