Multicenter Comparison of Seven 25Oh Vitamin D Automated Immunoassays / Multicentrično Poređenje Sedam Automatizovanih Imunoeseja Za 25Oh Vitamin D

Author:

Lippi Giuseppe,Salvagno Gian Luca,Fortunato Antonio,Dipalo Mariella,Aloe Rosalia,Rin Giorgio Da,Giavarina Davide

Abstract

SummaryBackground: The measurement of 25OH vitamin D continues to grow in clinical laboratories. The aim of this multicenter study was to compare the results of seven automated commercial immunoassays with a reference HPLC technique.Methods: One hundred and twenty consecutive outpatient serum samples were centrifuged, divided in aliquots, frozen and shipped to the participating laboratories. 25OH Vita - min D was measured with a reference HPLC system and with seven automated commercial immunoassays (Roche Cobas E601, Beckman Coulter Unicel DXI 800, Ortho Vitros ES, DiaSorin Liaison, Siemens Advia Centaur, Abbott Architect i System and IDS iSYS).Results: Compared to the reference method, the regression coefficients ranged from 0.923 to 0.961 (all p<0.001). The slope of Deming fit ranged from 0.95 to 1.06, whereas the intercept was comprised between -15.2 and 9.2 nmol/L. The bias from the reference HPLC technique varied from 14.5 to 8.7 nmol/L. The minimum performance goal for bias was slightly exceeded by only one immunoassay. The agreement between HPLC and the different immunoassays at 50 nmol/L 25OH Vitamin D varied between 0.61 and 0.85 (all p<0.001). The percentage of samples below this cut-off was significantly different with only one immunoassay.Conclusions:The excellent correlation with the reference HPLC technique attests that all seven automated immuno - assays may be reliably used for routine assessment of 25OH-D in clinical laboratories. The significant bias among the different methods seems mostly attributable to the lack of standardization and calls for additional efforts for improving harmonization of 25OH-D immunoassays.

Publisher

Centre for Evaluation in Education and Science (CEON/CEES)

Reference19 articles.

1. Comparison of three different methods for vitamin determination and vitamin D status in general population;Jovičić;J Med Biochem,2012

2. thrombosis and hemostasis : more than skin deep;Targher;Semin Thromb Hemost,2012

3. Performance evaluation of Siemens ADVIA Centaur and Roche MODULAR Analytics Total OH Vitamin D assays;Chen;Clin Biochem,2012

4. Four automated OH total vitamin D immunoassays and commercial liquid chromatography tandem - mass spectrometry in Finnish population;Koivula;Clin Lab,2013

5. An assessment of hydroxy vitamin D measurements in comparability studies conducted by the Vitamin D Metabolites Quality Assurance Program;Bedner;Clin Chim Acta,2013

Cited by 5 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3