Gender- and age-specific reference intervals of common biochemical analytes in chinese population – derivation using real laboratory data

Abstract

Summary Background Indirect sampling methods are not only inexpensive but also efficient for establishing reference intervals (RIs) using clinical data. This study was conducted to select fully normal records to establish age- and gender-specific RIs for common biochemical analytes by laboratory data mining. Methods In total, 280,206 records from 2014 to 2018 were obtained from Peking Union Medical College Hospital. Common biochemical analytes [total protein, albumin, total bilirubin (TBil), direct bilirubin (DBil), alanine aminotransferase (ALT), glutamyltranspeptidase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), potassium, sodium, chlorine, calcium, urea, glucose, uric acid (UA), inorganic phosphorus, creatinine (Cr), total cholesterol, triglyceride, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol] were measured using an automatic analyzer. Sources of variation were identified by multiple regression analysis. The 2.5th and 97.5th percentiles were calculated as the lower and upper limits of the RIs, respectively. Results Metode indirektnog uzorkovanja nisu samo jeftine, već su i efikasne za uspostavljanje referentnih intervala (RI) korišćenjem velike količine kliničkih podataka. Ova studija je sprovedena sa ciljem da se odaberu potpuno normalni pojedinačni zapisi da bi se utvrdio RI za starost i pol za zajedničke biohemijske analite pomoću laboratorijski dobijenih podataka. Conclusions Ukupno je uzeto 280.206 pojedinačnih rezultata u periodu od 2014. do 2018. godine iz bolnice Peking Union Medical College-a. Uobičajeni biohemijski analiti [ukupni protein, albumin, ukupni bilirubin (TBil), direktni bilirubin (DBil), alanin aminotransferaza (ALT), glutamiltranspeptidaza (GGT), alkalna fosfataza (ALP), aspartat aminotransferaza (AST), laktat dehidrogenaza (LAT) kalijum, natrijum, hlor, kalcijum, ureja, glukoza, mokraćna kiselina (UA), neorganski fosfor, kreatinin (Cr), ukupni hole sterol, trigliceridi, lipoprotein holesterola visoke gustine i lipoprotein holesterola niske gustine] mereni su korišćenjem automatskog analizatora. Izvori varijacije identifikovani su višestrukom regresionom analizom. Postoci (2,5 i 97,5) su izračunati kao donja i gornja granica RI, svaki ponaosob.