New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA
Author:
Chaouadi M.1, Scarpa F.2, Azzena I.23, Cossu P.3, Harhoura K.4, Aissi M.4, Tazerouti F.1, Garippa G.3, Merella P.3, Casu M.3, Sanna D.2
Affiliation:
1. Laboratory of Biodiversity and Environment: Interactions and Genomes, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, BP 32 , El Alia Bab Ezzouar , Algiers , Algeria 2. Department of Biomedical Sciences, University of Sassari , 07100 Sassari , Italy 3. Department of Veterinary Medicine, University of Sassari , 07100 Sassari , Italy 4. Laboratory of Animal Health and Production, Superior National Veterinary School Rabie Bouchama , Street Issad Abbes , Oued Smar , Algiers , Algeria
Abstract
Summary
This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 F. hepatica flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among F. hepatica isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of F. hepatica are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes’ worldwide distribution.
Publisher
Walter de Gruyter GmbH
Subject
Animal Science and Zoology,Parasitology
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