Natural rules for Arabidopsis thaliana pre-mRNA splicing site selection-Reference-Cited by-同舟云学术

Natural rules for Arabidopsis thaliana pre-mRNA splicing site selection

Published:2012-08-01 Issue:4 Volume:7 Page:620-625
ISSN:2391-5412
Container-title:Open Life Sciences
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Author:

Wu Ning¹,Matand Kanyand,Wu Huijuan²,Li Baoming²,Love Kayla³,Stoutermire Brittany²,Wu Yanfeng⁴

Affiliation:

1. 1Center for Biotechnology Research and Education, Langston University, Langston, Oklahoma, 73050, USA

2. 3Department of Biology, Beijing Center for Physical and Chemical Analysis, 100089, Beijing, China

3. 4Department of Chemistry, School of Arts and Science, Langston University, Langston, Oklahoma, 73050, USA

4. 2Department of Biology, School of Arts and Science, Langston University, Langston, Oklahoma, 73050, USA

Abstract

AbstractThe accurate prediction of plant pre-mRNA splicing sites has been studied extensively. The rules for plant pre-mRNA splicing still remain unknown. This study, based on confirmed sequence data, systematically analyzed all expressed genes on Arabidopsis thaliana chromosome IV to quantitatively explore the natural splicing rules. The results indicated that defining Arabidopsis thaliana pre-mRNA splicing sites required a combination of multiple factors including (1) relative conserved consensus sequence at splicing site; (2) individual nucleotide distribution pattern in 50 nucleotides up- and down-stream regions of splicing site; (3) quantitative analysis of individual nucleotide distribution by using the formulations concluded from this study. The combination of all these factors together can bring the accuracy of Arabidopsis thaliana splicing site recognition over 99%. The results provide additional information to the future of plant pre-mRNA splicing research.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Link

http://link.springer.com/content/pdf/10.2478/s11535-012-0060-1.pdf

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