A way to identify archaellins in Halobacterium salinarum archaella by FLAG-tagging-Reference-Cited by-同舟云学术

A way to identify archaellins in Halobacterium salinarum archaella by FLAG-tagging

Published:2013-09-01 Issue:9 Volume:8 Page:828-834
ISSN:2391-5412
Container-title:Open Life Sciences
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Author:

Beznosov Sergei¹,Pyatibratov Michael¹,Veluri Pavan²,Mitra Sagar²,Fedorov Oleg¹

Affiliation:

1. 1202Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Russia

2. 2202Department of Energy Science and Engineering, Indian Institute of Technology, 400076, Mumbai, India

Abstract

AbstractIn the current study, haloarchaea Halobacterium salinarum cells were transformed individually with each of the modified archaellin genes (flaA1, flaA2 and flaB2) containing an oligonucleotide insert encoding the FLAG peptide (DYKDDDDK). The insertion site was selected to expose the FLAG peptide on the archaella filament surface. Three types of transformed cells synthesizing archaella, containing A1, A2, or B2 archaellin modified with FLAG peptide were obtained. Electron microscopy of archaella has demonstrated that in each case the FLAG peptide is available for the specific antibody binding. It was shown for the first time that the B2 archaellin, like archaellins A1 and A2, is found along the whole filament length.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Link

http://link.springer.com/content/pdf/10.2478/s11535-013-0202-0.pdf

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