A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols-Reference-Cited by-同舟云学术

A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols

Published:2013-01-01 Issue:1 Volume:8 Page:48-53
ISSN:2391-5412
Container-title:Open Life Sciences
language:
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Author:

Attard Everaldo¹

Affiliation:

1. 1Institute of Earth Systems, Division of Rural Sciences and Food Systems, University of Malta, MSD 2080, Msida, Malta

Abstract

AbstractSeveral methods have been described for the determination of phenolic compounds in animal and plant products using the Folin-Ciocalteu (FC) assay. Most of these methods describe the use of this reagent and sodium carbonate in spectrophotometric methods. The macro FC assay was compared with two micro FC assays carried out on a microplate reader. Excellent correlation was obtained among the three assays with a molar extinction coefficient of 5.228±0.187x103 M−1 cm−1. The micro assay may serve as a high throughput method for the rapid determination of polyphenols in various samples.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Link

http://link.springer.com/content/pdf/10.2478/s11535-012-0107-3.pdf

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