NMDA-induced stimulation of glycolysis in developing hippocampal cell cultures-Reference-Cited by-同舟云学术

NMDA-induced stimulation of glycolysis in developing hippocampal cell cultures

Published:2009-03-01 Issue:1 Volume:4 Page:50-57
ISSN:2391-5412
Container-title:Open Life Sciences
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Author:

Luengviriya Jiraporn,Helmeke Carina¹,Braun Katharina¹,Hauser Marcus²,Mair Thomas²

Affiliation:

1. 3Department of Zoology/Developmental Neurobiology, Otto-von-Guericke University, 39120, Magdeburg, Germany

2. 1Insitute of Experimental Physics, Department of Biophysics, Otto-von-Guericke University, 39106, Magdeburg, Germany

Abstract

AbstractDevelopmental changes in energy metabolism of primary hippocampal cell cultures from newborn rats were investigated during the first 3 weeks. These changes were measured by intensity of and number of cells exhibiting NAD(P)H fluorescence in response to NMDA-induced activation of neuronal activity. We observed gradual changes of stimulation-evoked NAD(P)H signaling over the first 3 weeks, such that at day 7 and 16, this stimulation is minimal, while at 5 and 12 days, it is maximal. These results describe a biphasic pattern that was similar to earlier findings from experiments investigating developmental changes in population spike amplitudes or glutamate release in young rats. Inhibition of mitochondrial respiration by KCN revealed that the NMDA-evoked stimulation of energy metabolism is mainly due to increased glycolytic activity.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Link

http://link.springer.com/content/pdf/10.2478/s11535-008-0052-3.pdf

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