Production of hairy root cultures of lettuce (Lactuca sativa L.)-Reference-Cited by-同舟云学术

Production of hairy root cultures of lettuce (Lactuca sativa L.)

Published:2014-12-01 Issue:12 Volume:9 Page:1196-1205
ISSN:2391-5412
Container-title:Open Life Sciences
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Author:

Vojin Tadić¹,Snežana Milošević¹,Aleksandar Cingel¹,Marija Petrić¹,Milana Trifunović¹,Dragana Antonić¹,Jovan Tadić²,Angelina Subotić¹

Affiliation:

1. 1Department of Plant Physiology, Institute for Biological Research „Siniša Stanković“, University of Belgrade, 11060, Belgrade, Serbia

2. 2Department of Global Ecology, Carnegie Institution for Science, Stanford, CA, 94305, USA

Abstract

AbstractHairy root cultures of lettuce (Lactuca sativa L.) were obtained by inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs. Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS. Approximately in 96.7% cvs. Nansen and in 91.2% Ljubljanska ledenka inoculated explants produced hairy root when they were incubated on Murashige and Skoog (MS) half-strength medium without plant growth regulators. A total of 54% of all hairy root cultures expressed GUS activity. Every hairy root represented an independent transformation event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times the biomass of control root). A PCR analysis of the genomic DNA confirmed the presence of marker and target genes in 15 hairy roots examined.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Link

http://link.springer.com/content/pdf/10.2478/s11535-014-0351-9.pdf

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