Genetic characterisation of centuries-old oak and linden trees using SSR markers-Reference-Cited by-同舟云学术

Genetic characterisation of centuries-old oak and linden trees using SSR markers

Published:2022-03-01 Issue:1 Volume:64 Page:58-68
ISSN:2199-5907
Container-title:Folia Forestalia Polonica
language:en
Short-container-title:

Author:

Bilous Svitlana¹²,Prysiazhniuk Larysa³,Chernii Snizhana³,Melnyk Serghii³,Marchuk Yurii¹,Likhanov Artur¹²

Affiliation:

1. National University of Life and Environmental Science of Ukraine , Dendrology and Forest Tree Breeding Department , General Rodimtsev 19 , Kyiv , Ukraine , phone: +380501399386

2. Institute for Evolutionary Ecology NAS of Ukraine, Department of Dendrology and Park Learning , Lebedeva 37 , Kyiv , Ukraine

3. Ukrainian Institute for Plant Variety Examination , Ukraine

Abstract

Abstract The main goal of this study was to identify the unique gene pool of old and historically valuable Quercus robur L. and Tilia cordata L. to be able to characterise their genetic diversity in order to determine the polymorphism by expressed sequence tag-single sequence repeat (EST-SSR) markers and identify the most valuable specimens. Morphological description, molecular genetic analysis, and statistical analysis were used in studies. The genetic distances between old-value trees of different Quercus L. and Tilia L. were determined based on EST-SSR markers and morphological characteristics. Using polymerase chain reaction (PCR), alleles of the expected size were obtained. It was determined that four to eight alleles were obtained by seven SSR markers in the studied Q. robur L. samples. According to the calculated value of the locus polymorphism index (polymorphism information content [PIC]), the most polymorphic was the marker SSRQrZAG 65; the PIC was 0.84. The lowest value of PIC was observed in the marker SSRQrZAG 11; the PIC was 0.69. Intragenetic polymorphism was detected for all studied markers. Among the studied samples of linden, two to five alleles were identified. It was found that the highest value of PIC was obtained for the marker Ts920 – 0.72. The least polymorphic was the marker Ts927 (PIC was 0.28), which is not only due to the small number of alleles, but also their uneven distribution in the sample. Intragenetic polymorphism was detected in four of the six markers analysed for T. cordata L. In this study, polymorphism was detected in all studied samples of Q. robur L. and T. cordata L., which allows to assess their genetic diversity based on the distribution of alleles.

Publisher

Walter de Gruyter GmbH

Subject

Ecology,Forestry

Link

https://www.sciendo.com/pdf/10.2478/ffp-2022-0006

Reference39 articles.

1. Barreneche, T. et al. 1998. A genetic linkage map of Quercus robur L. (pedunculate oak) based on RAPD, SCAR, microsatellite, minisatellite, isozyme and 5S rDNA markers. Theoretical and Applied Genetics, 97 (7), 1090–1103.

2. Bilous, S. 2013. Features of obtaining aseptic culture of a centuries-old tree Tilia cordata Mill. “linden T.G. Shevchenko”. Scientific Bulletin of the National University of Life and Environmental Sciences of Ukraine “Forestry and ornamental horticulture”, 187 (3), 213–217. http://nbuv.gov.ua/j-pdf/nvnau_lis_2013_187_3_35.pdf

3. Bilous, S. 2018. Types of tissues differentiation of centuries-old oak T.G. Shevchenko in vitro. In: Materials of International Scientific and Practical Conference: Floristic and coenotic diversity in the restoration, preservation and protection of flora, 23–25 April 2018, Kyiv, 156–158.

4. Bilous, A., Matsala, M., Radchenko, V., Matiashuk, R. Boyko, S., Bilous, S. 2019. Coarse woody debris in mature oak stands of Ukraine: carbon stock and decomposition features. Forestry Ideas, 25 (1), 196–219.

5. Chornobrov, O., Bilous, S., Chornobrov, O., Manko, M. 2019. Peculiarities of morphogenesis of the endangered species of willow (Salix spp.) in vitro. Biologija, 65 (1), 48–55.