Nematode Genome Announcement: A Draft Genome of Seed Gall Nematode, Anguina tritici

Author:

Singh Ashish Kumar12,Das Antara3,Joshi Ila3,Kumar Manish1,Kohli Deshika3,Pankaj 1,Gaikwad Kishor3,Jain Pradeep Kumar3,Sirohi Anil1

Affiliation:

1. 1 Division of Nematology , ICAR-Indian Agricultural Research Institute , Pusa Campus , New Delhi - , India .

2. 2 Division of Crop Protection, ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan , Almora , Uttarakhand , India .

3. 3 ICAR-National Institute for Plant Biotechnology , Pusa Campus , New Delhi , , India .

Abstract

Abstract Anguina tritici is the first plant-parasitic nematode described in literature, dating back to the year 1743. It is responsible for causing earcockle (seed gall) and tundu diseases in wheat and rye. Notably, this nematode has been observed to survive in an anhydrobiotic state for up to 32 years within wheat seed galls. These exceptional characteristics have inspired the sequencing of the A. tritici genome. In this study, we present the initial draft genome of A. tritici, obtained using the Illumina MiSeq platform with coverage of 60-fold. The genome is estimated to have a size of 164 Mb and comprises 39,965 protein-coding genes, exhibiting a GC content of 39.1%. The availability of this genome data will serve as a foundation for future functional biological investigations, particularly for genes whose functions remain unknown to this day.

Publisher

Walter de Gruyter GmbH

Reference24 articles.

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2. Wasmuth, J., Schmid, R., Hedley, A., and Blaxter, M. 2008. On the extent and origins of genic novelty in the phylum Nematoda. PLOS Neglected Tropical Diseases 2(7):e258.

3. Limber, D.P. (1973). Notes on the longevity of Anguina tritici (Steinbuch, 1799) Filipjev, 1936, and its ability to invade wheat seedlings after thirty-two years of dormancy. Proceedings of the Helminthological Society, Volume 40, Number 2, July 1973.

4. Joshi, I., Kumar, A., Singh, A. K., Kohli, D., Raman, K. V., Sirohi, A., Chaudhury, A., and Jain, P. K. 2019. Development of nematode resistance in Arabidopsis by HD-RNAi-mediated silencing of the effector gene Mi-msp2. Scientific Reports 9:17404. https://doi.org/10.1038/s41598-019-53485-8.

5. Thomas, J. C., Khoury, R., Neeley, C. K., Akroush, A. M., and Davies, E. C. 1997. A fast CTAB method of human DNA isolation for polymerase chain reaction applications. Biochemical Education 25(4):233–235.

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