Vertical Migration of Second-stage Juveniles of <i>Meloidogyne enterolobii</i> as Influenced by Temperature and Host-Reference-Cited by-同舟云学术

Vertical Migration of Second-stage Juveniles of Meloidogyne enterolobii as Influenced by Temperature and Host

Published:2024-03-01 Issue:1 Volume:56 Page:
ISSN:2640-396X
Container-title:Journal of Nematology
language:en
Short-container-title:

Author:

Oliveira Ana Karina S.¹,Pedrosa Elvira M. R.¹,Leitão Diego A. H. S.²³,Brito Janete A.⁴,Silva Ênio F. de F.¹,Dickson Donald W.³

Affiliation:

1. Agricultural Engineering Department , Federal Rural University of Pernambuco , Recife , Pernambuco , Brazil .

2. Soil, Water and Ecosystem Sciences Department , University of Florida , Gainesville , FL , USA . (Current address)

3. Entomology and Nematology Department , University of Florida , Gainesville , FL , USA .

4. Florida Department of Agriculture and Consumer Services , Division of Plant Industry , Gainesville , FL , USA .

Abstract

Abstract Infective second-stage juveniles (J2) of Meloidogyne spp. migrate towards host roots, which depends on several factors, including root exudates and soil temperature. Although Meloidogyne enterolobii is a highly virulent nematode that affects major agricultural crops worldwide, there is limited ecological data about it. The objective of this study was to determine the J2 migration pattern vertically in 14-cm long segmented soil columns towards tomato (Solanum lycopersicum) and marigold (Tagetes patula) roots, each grown at two soil temperatures (20 or 26ºC). Bottomless cups with tomatoes or marigolds were attached to the top of each column; cups with no plants were used as untreated controls. Juveniles (1,000/column) were injected into a hole located 1 cm from the bottom of each column. The apparatuses were placed in growth chambers at 20 or 26ºC, and J2 were allowed to migrate for 3, 6, 9, or 12 days after injection (DAI). At each harvest, J2 were extracted from each ring of the columns and counted to compare their distribution, and root systems were stained to observe root penetration. M. enterolobii migrated over 13 cm vertically 3 DAI regardless of temperature, even without plant stimuli. The vertical migration was greater at 26ºC, where 60% of active J2 were found at distances >13 cm at 12 DAI. Temperature did not affect root penetration. Overall, a greater number of J2 was observed in tomato roots, and root penetration increased over time.

Publisher

Walter de Gruyter GmbH

Link

https://www.sciendo.com/pdf/10.2478/jofnem-2024-0012

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