Lactobacillus Spp. strains isolation, identification, preservation and quantitative determinations from the intestinal content and faeces of weaned piglets

Abstract

Abstract The study aimed to isolate, identify, preserve and evaluate the quantitative level of the Lactobacillus strains from gut content and faeces of weaned piglets, 30-107 days old; to test the viability of these strains preserved at 4°C and room temperature. Lactobacillus strains were isolated, phenotypically identified and preserved from gut content and faeces of 20 weaned piglets. Identification was performed by morphological, cultural and biochemical character examination, using apiwebTM and ABIS online software. Lactobacillus spp. from intestinal content and faeces (106 – 109 CFU/g) and the viability of strains preserved at 4°C and at room temperature were also determined (from 38 days to 4 months). Twenty-six strains of L. acidophilus, L. fermentum, L. plantarum, L. salivarius and L. delbrueckii ssp. delbrueckii, from gut content and faeces of weaned piglets were isolated, phenotypic identified and preserved. Of these, L. fermentum, L. delbrueckii ssp. delbrueckii and L. acidophilus biotype 2 isolates were technologically and ecologically suitable for continuing the testing of probiotic traits.