Viability of bovine in vitro matured oocytes following ultra-rapid vitrification-Reference-Cited by-同舟云学术

Viability of bovine in vitro matured oocytes following ultra-rapid vitrification

Published:2022-03-01 Issue:1 Volume:10 Page:1-6
ISSN:2544-3577
Container-title:Medical Journal of Cell Biology
language:en
Short-container-title:

Author:

Dujíčková Linda¹²,Olexiková Lucia¹,Kubovičová Elena¹,Bezdíček Jiří³,Ješeta Michal⁴⁵,Makarevich Alexander¹

Affiliation:

1. National Agricultural and Food Centre (NPPC), Research Institute for Animal Production Nitra , Lužianky , Slovak Republic

2. Department of Botany and Genetics, Faculty of Natural Sciences , Constantine the Philosopher University in Nitra , Nitra , Slovak Republic

3. Department of Zoology , Palacký University Olomouc , Olomouc , Czech Republic

4. Department of Obstetrics and Gynecology , University Hospital and Masaryk University , Brno , Czech Republic

5. Department of Veterinary Sciences , Czech University of Life Sciences in Prague , Prague , Czech Republic

Abstract

Abstract The aim of the study was to examine viability of cattle oocytes after cryopreservation. Oocytes after in vitro maturation (IVM) were vitrified in minimum volume on the nickel electron microscopy grids by ultra-rapid cooling technique. After warming and subsequent in vitro fertilization the presumptive zygotes were cultured to reach the stage of the blastocyst (Bl). Several devitrified oocytes were processed for electron microscopy assay. Although, embryo cleavage and Bl percentages in the vitrified group were slightly lower than in the control group (P < 0.05), the Bl total cell number (TCN), apoptosis and dead cell percentages did not differ between both groups. However, significant difference was found between day 7 (D7) and day 8 (D8) Bl in the TCN in control (108.0 vs. 90.5) and vitrified group (103.75 vs 98.14). Electron microscopy of frozen oocytes revealed slight reversible injuries in mitochondria and the smooth endoplasmic reticulum (SER), nevertheless, the development of devitrified oocytes to the Bl stage was comparable to those in fresh oocytes. In conclusion, higher proportion of slower developing Bl (D8) compared to D7 Bl may be related to the mentioned minor damages of some organelles in vitrified oocytes.

Publisher

Walter de Gruyter GmbH

Subject

Cell Biology,Molecular Biology

Link

https://www.sciendo.com/pdf/10.2478/acb-2022-0001

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