Author:
Auriemma Clementina,Lucibelli Maria,Borriello Giorgia,Carlo Esterina,Martucciello Alessandra,Schiavo Lorena,Gallo Amalia,Bove Francesca,Corrado Federica,Girardi Santa,Amoroso Maria,Ďegli Uberti Barbara,Galiero Giorgio
Abstract
AbstractThe seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes.
Reference20 articles.
1. Neosporosis in water buffalo Bubalus bubalis ) in Southern Italy;Guarino;Veterinary Parasitology,2000
2. Neospora caninum : specific oligonucleotide primers for the detection of brain cyst DNA of experimentally infected nude mice by the polymerase chain reaction http dx doi org;Yamage;Journal of Parasitology,1996
3. Molecular evidence for the existence of additional members of the order Chlamydiales;Ossewaarde;Microbiology,1999
4. Pestiviruses isolated from pigs , cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis http dx doi org;Vilcek;Archives of Virology,1994
5. Multiplex PCR for direct identification of Campylobacter spp in human and chicken stools;Al Amri;Journal of Medical Microbiology,2007
Cited by
21 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献