Genotype Variation of Polyphenol Content and Antioxidant Activity of Artemisia balchanorum Krasch. × Artemisia taurica Willd
Author:
Vergun Olena1, Svydenko Liudmyla2, Grygorieva Olga1, Hauptvogel Pavol3, Brindza Ján4
Affiliation:
1. 1 M.M. Gryshko National Botanical Garden of the National Academy of Ukraine , Kyiv , Ukraine 2. 2 Institute of Climate Smart Agriculture of the National Academy of Agrarian Sciences of Ukraine , Kyiv , Ukraine 3. 3 National Agricultural and Food Center , Slovak Republic 4. 4 Slovak University of Agriculture in Nitra , Nitra , Slovak Republic
Abstract
Abstract
The results of seasonal variation of total polyphenols (TPC), flavonoids (TFC) and phenolic acid (TPAC) accumulation and antioxidant activity of Artemisia balchanorum Krasch. × Artemisia taurica Willd. genotypes from the Kherson Oblast of Ukraine represented in this study. The total polyphenol content was identified by the Folin-Ciocalteu method and ranged from 20.51 to 132.52 mg GAE/g (gallic acid equivalent). The flavonoid content conducted with aluminum chloride reagent ranged from 8.16 to 70.77 mg QE/g (quercetin equivalent) and phenolic acid content determined with Arnova reagent ranged from 6.26 to 38.67 mg CAE/g (caffeic acid equivalent) depending on genotype and stage of growth. The antioxidant activity of investigated genotypes determined by the DPPH (2.2-diphenyl-1-picrylhydrazyl) method (FRSA) varied from 4.68 to 10.38 mg TE/g (Trolox equivalent), molybdenum-reducing power of extracts detected by the phosphomolybdenum method (MRP) and was from 41.43 to 209.93 mg TE/g depending on genotype. The total polyphenol content, flavonoids and antioxidant activity by the phosphomolybdenum method increased from spring vegetation to flowering. The accumulation of phenolic acids and antioxidant activity by the DPPH method was uneven and depended on genotype and stage of growth. It was found a weak correlation between FRSA and TPC (r = 0.189), TFC (r = 0.211), and TPAC (r = 0.111) for genotypes. A strong correlation was found between MRP and TPC (r = 0.703), and TFC (r = 0.657). A moderate correlation was detected between MRP and TPAC (r = 0.481) for genotypes. The obtained data can be used for further breeding work to get genotypes with high antioxidant activity.
Publisher
Walter de Gruyter GmbH
Subject
Horticulture,Plant Science,Soil Science,Agronomy and Crop Science
Reference58 articles.
1. Ahmed, M., Peiwen, Q., Gu, Z., Liu, Y., Sikandar, A., Hussain, D., Javeed, A., Shafi, J., Iqbal, M. F., An, R., Guo, H., Du, Y., Wang, W., Zhang, Y., and Ji, M. (2020). Insecticidal activity and biochemical composition of Citrullus colocynthis, Cannabis indica and Artemisia argyi extracts against cabbage aphid (Brevicoryne brassicae L.). Scientific Reports, 10(1), 522. DOI:10.1038/s41598-019-57092-5. 2. Alam, N. Md., Bristi, N. J. and Rafiquzzaman, Md. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21(2), 143 – 152. DOI:10.1016/j.jsps.2012.05.002. 3. Ansari, M. T., Saify, Z. S., Sultana, N., Ahmad, I., Saeed-Ul-Hassan, S., Tariq, I., and Khanum, M. (2013). Malaria and artemisinin derivatives: an updated review. Mini-Reviews in Medicinal Chemistry, 13(13), 1879 – 1902. DOI:10.2174/1 389557511136660097. 4. Árvay, J., Hauptvogl, M., Ivanisova, E., Tidil’ová, I., Hrstková, M., Bajčan, D., and Lazor, P. (2017). Methylxanthines and catechines in different teas (Camellia sinensis L. Kuntze) – influence on antioxidant properties. Potravinarstvo Slovak Journal of Food Science, 11(1), 510 – 516. DOI:10.5219/796. 5. Bisht, D., Kumar, D., Kumar, D., Dua, K., and Chellappan, D. K. (2021). Phytochemistry and pharmacological activity of the genus Artemisia. Archives of Pharmacological Research, 44(5), 439 – 474. DOI:10.1007/s12272-021-01328-4.
|
|