Variations in the Expression Pattern of HSP27 and MSK1 Genes During the Development of Prehierarchical Follicles in the Zi Geese (Anser Cygnoides)-Reference-Cited by-同舟云学术

Variations in the Expression Pattern of HSP27 and MSK1 Genes During the Development of Prehierarchical Follicles in the Zi Geese (Anser Cygnoides)

Published:2020-01-01 Issue:1 Volume:20 Page:43-53
ISSN:2300-8733
Container-title:Annals of Animal Science
language:en
Short-container-title:

Author:

Sello Cornelius Tlotliso¹,Liu Chang¹,Lu Hongtao¹,Wang Ziqiu¹,Msuthwana Petunia¹,Tyasi Thobela Louis¹,Sun Yue¹,Liu Jing¹,Xu Chenguang¹,Zhou Yuxuan¹,Li Shengyi¹,Yang Hui¹,Sui Yujian¹,Hu Jingtao²,Sun Yongfeng¹³

Affiliation:

1. Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology , Jilin Agricultural University , Xincheng Street, No: 2888, Changchun 130118 , Jilin, China

2. College of Animal Science and Technology, Jilin Provincial Engineering Research Centre of Animal Probiotics , Jilin Agricultural University , Changchun 130118 , Jilin, China

3. Key Laboratory for Animal Production, Product Quality and Safety of Ministry of Education , Jilin Agricultural University , Xincheng Street, No: 2888, Changchun 130118 , Jilin, China

Abstract

Abstract The p38MAPKs (mitogen-activated protein kinases) signaling contributes a pivotal role in mammalian ovarian follicular development; however, the knowledge regarding their expression in geese remains unresolved. The objective of the current study was to determine the spatio-temporal expression of heat shock protein 27 (HSP27) and mitogen- and stress-activated protein kinase 1 (MSK1) genes in the prehierarchical follicles during geese ovarian development. The prehierarchical follicles samples were harvested from 35- to 37-week-old healthy laying geese. HSP27 and MSK1 relative expression in various sized prehierachical follicles was detected by real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting. Follicular wall localization of HSP27 and MSK1 was examined by using immunohistochemistry. Our results at mRNA level indicated that HSP27 was highly expressed in middle white follicles whereas MSK1 was predominantly expressed in small white follicles. The western blotting results for HSP27 and MSK1 were inconsistent with the RT-qPCR results in various stages of prehierachical follicular development but noticeably, HSP27 proteins were still expressed more in middle white follicles while MSK1 proteins were more abundant in primary follicles. At different stages of prehierarchical development, immunodetections in the granulosa and theca cells revealed that HSP27 was intensively localized in middle white follicles while strong detections of MSK1 were observed in large white follicles. These results indicate HSP27 and MSK1 might be associated to the key regulators of folliculogenesis in geese.

Publisher

Walter de Gruyter GmbH

Link

https://www.sciendo.com/pdf/10.2478/aoas-2019-0048

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