Impact of un-polymerized acrylamide monomer residues onto protein identification by MALDI TOF MS

Author:

Talian Ivan1,Kováčová Veronika1,Petrovič Marián1,Sabo Ján1

Affiliation:

1. 1Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia

Abstract

AbstractPolyacrylamide gel electrophoresis is a powerful tool for protein mixture separation frequently used in proteomic studies. This article describes the problems which can arise during the identification process of separated proteins by matrix assisted laser desorption/ionization mass spectrometry. Presence of residual acrylamide monomers in the peptide samples leads to a significant peptide signal suppression. In the case of protein prohibitin, isolated from the MCF 7 breast cancer cell line, the peptide signal suppression was observed in 50% of all detected peptides.

Publisher

Walter de Gruyter GmbH

Subject

Materials Chemistry,General Chemistry

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