PCR based prevalence study of Francisella tularensis in Kharkiv, Dnipropetrovsk, and Mykolaiv oblasts during 2015–2018
Author:
Zlenko Oksana Borysivna1, Tkach Gennadiy Evgenievich2, Sukhorukova Anna Borysivna2, Kylypko Lyudmila Vitaliivna2, Machota Lubov Stepanivna2, Ignatenkov Oleksandr Stefanovych3, Vinokurova Kateryna Volodymyrivna3, Shamychkova Galyna Rostyslavivna4, Shtepa Oleksandr Pymonovych4, Rezvykh Valentyna Gennadievna4, Schwarz Julia5, Duerr Angela5, Popp Claudia5, Buttlar Heiner von5, Wolfel Roman5, Solodiankin Oleksii Sergiiovych1, Gerilovych Anton Pavlovych1
Affiliation:
1. Department of Molecular Epizootology and Diagnostics, National Scientific Center Institute of Experimental and Clinical Veterinary Medicine of the NAAS of Ukraine , 61023 Kharkiv , Ukraine 2. SI Kharkiv Oblast Laboratory Centre of the MoH of Ukraine , Pomirky, 61070 Kharkiv , Ukraine 3. SI Mykolaiv Oblast Laboratory Centre of the MoH of Ukraine , 54058 Mykolaiv , Ukraine 4. SI Dnipropetrovsk Oblast Laboratory Centre of the MoH of Ukraine , 49064 Dnipro , Ukraine 5. Department of Bacteriology and Toxicology, Bundeswehr institute of Microbiology , 80937 Munich , Germany
Abstract
Abstract
Introduction
Tularaemia is a zoonotic disease caused by the gram-negative bacterium Francisella tularensis, which is endemic to Ukraine. The aim of this work was to provide screening of different field samples (rodent tails, ticks, pellets, water, and hay) to obtain an actual picture of the tularaemia epizootic situation in the Kharkiv, Dnipropetrovsk, and Mykolaiv oblasts.
Material and Methods
Samples were collected using the flag method (for ticks) and break-back traps (for rodents). Also, hay, water and owl pellets were collected for study. The F. tularensis genetic material in samples was detected using a 16S qPCR.
Results
It was found that in Kharkiv oblast, 23% of collected samples were positive for F. tularensis, in Dnipropetrovsk oblast 1.9%, and in Mykolaiv oblast 0.4%.
Conclusion
Among the sample types, 34.7% of ticks, 1.8% of rodents, and 36.4% of pellets were positive for F. tularensis. The most frequent carriers of F. tularensis were the D. reticulatus and I. ricinus ticks (74.2% and 29.3%, respectively, of positive results).
Publisher
Walter de Gruyter GmbH
Subject
General Veterinary
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