Affiliation:
1. Department of Anatomy , , Van , Turkey
2. Department of Pathology, Faculty of Veterinary Medicine, University of Van Yüzüncü Yıl , , Van , Turkey
3. Department of Pathology, Faculty of Veterinary Medicine, University of Hatay Mustafa Kemal , , Hatay , Turkey
4. Department of Medical Physics, Faculty of Medicine, University of Van Yüzüncü Yıl , , Van , Turkey
Abstract
Abstract
Introduction
This study determined the presence of nitric oxide synthesis isoforms (nNOS, iNOS, and eNOS) in thoracic spinal cord segments and nodose ganglia of rats with gamma-irradiated livers.
Material and Methods
Male rats (n = 32) were divided into equal groups A, B, C, and D. In group A, the controls, no radiation was applied, while groups B, C, and D received 10 Gy of ionising gamma radiation. The rats of group B were euthanized at the end of the first day (d1), those of group C on the second day (d2), and those of group D on the third day (d3). The liver, spinal cord segments, and nodose ganglion tissues were dissected and fixed, and the liver sections were examined histopathologically. The other tissues were observed through a light microscope.
Results
Regeneration occurred at the end of d3 in hepatocytes which were radiation-damaged at the end of d1 and d2. On d1, some nNOS-positive staining was found in the neuronal cells of laminae I–III of the spinal cord and in neurons of the nodose ganglion, and on d3, some staining was observed in lamina X of the spinal cord, while none of note was in the nodose ganglion. Dense iNOS-positive staining was seen on d1 in the ependymal cells of the spinal cord and in the glial cells of the nodose ganglion, and on d3, there was still considerable iNOS staining in both tissues. There was clear eNOS-positive staining in the capillary endothelial cells of the spinal cord and light diffuse cytoplasmic staining in the neurons of the nodose ganglion on d1, and on d3, intense eNOS-positive staining was visible in several endothelial cells of the spinal cord, while light nuclear staining was recognised in the neurons of the nodose ganglion.
Conclusion
The nNOS, iNOS, and eNOS isoforms are activated in the spinal cord and nodose ganglion of rats after ionising radiation insult to the liver.
Reference38 articles.
1. Alderton W.K., Cooper C.E., Knowles R.G.: Nitric oxide synthases: structure, function and inhibition. Biochem J 2001, 357, 593–615.
2. Alexander B.: The role of nitric oxide in hepatic metabolism. Nutrition 1988, 14, 376–390.
3. Alexandrova R., Mileva M., Zvetkova E.: Nitric oxide and cancer. Exp Pathol Parasitol 2001, 4, 13–18.
4. Aricioglu F.: Nitrik oksit ve santral sinir sistemi. Türk Farmakoloji Derneği, Farmakoloji Eğitim Seminerleri Proğramı, Nitrik Oksitin Farmakolojisi, Seminar Abstracts 2005, pp. 19–21.
5. Berthoud H.R.: Anatomy and function of sensory hepatic nerves. Anat Rec A Discov Mol Cell Evol Biol 2004, 280, 827–835.
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献