The first complete genome sequence and genetic evolution analysis of bovine norovirus in Xinjiang, China-Reference-Cited by-同舟云学术

The first complete genome sequence and genetic evolution analysis of bovine norovirus in Xinjiang, China

Published:2024-03-01 Issue:1 Volume:68 Page:1-8
ISSN:2450-8608
Container-title:Journal of Veterinary Research
language:en
Short-container-title:

Author:

Ma Zhigang¹²^ORCID,Jiang Qian¹²^ORCID,Quan Chenxi¹²^ORCID,Liu Lu¹²^ORCID,Zhang Zhonghua³^ORCID,Xie Jinxing¹²^ORCID,Zhao Lu⁴^ORCID,Zhong Qi⁵^ORCID,Yao Gang¹²^ORCID,Ma Xuelian¹²^ORCID

Affiliation:

1. College of Veterinary Medicine, Xinjiang Agricultural University , Urumqi Xinjiang , , China

2. Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animal (XJ-KLNDSCHA), Xinjiang Agricultural University , Urumqi Xinjiang , , China

3. Xinjiang Daolang Sunshine Agriculture and Animal Husbandry Technology Co., Ltd ., Kashgar Xinjiang , , China

4. Changji Prefecture Center for Animal Disease Control and Prevention , Changji Xinjiang , , China

5. Xinjiang Uygur Autonomous Region Animal Husbandry and Veterinary Society , Urumuqi Xinjiang , China

Abstract

Abstract Introduction Viruses are among the main pathogens causing diarrhoea in calves. The current study found that bovine norovirus (BNoV) is one of the principal viruses causing diarrhoea in calves in Xinjiang, China. Material and Methods A total of 974 calf faecal samples from six regions in Xinjiang were tested for BNoV using reverse-transcriptase PCR. The genomic characteristics of BNoV and the genetic evolution of the VP1 gene, protein three-dimensional structure characteristics and amino acid variation were analysed using bioinformatics methods. Results Epidemiological survey results showed that the infection rate of BNoV was 19.82%, and all samples tested positive in five regions. The results of the genetic evolution analysis showed that BNoV strains from Tacheng of northern Xinjiang and Kashgar of southern Xinjiang both belonged to the GIII.2 genotype of BNoV but were not on the same cluster of evolutionary branches. Additionally, the amino acid variation of the VP1 protein was not observed to significantly affect its spatial structure. Conclusion This study is the first to report the genetic characteristics of the BNoV complete genome sequence in Xinjiang and provides a scientific basis for BNoV vaccine development and pathogenesis research.

Publisher

Walter de Gruyter GmbH

Link

https://www.sciendo.com/pdf/10.2478/jvetres-2024-0005

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