Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus
Author:
Yin Mei1, Hu Dongfang1, Li Peng2, Kong Lingyun3, Ning Hongmei1, Yue Feng2, Jiang Jinqing1, Wang Xuannian2
Affiliation:
1. College of Animal Science and Technology, Henan Institute of Science and Technology , Xinxiang , Henan, 453003 , China 2. College of Life Science and Technology, Xinxiang University , Xinxiang , Henan, 453003 , China 3. Xinxiangxian Agriculture and Animal Husbandry Bureau (Xinxiangxian Forestry Bureau) , Xinxiang , Henan, 453700 , China
Abstract
Abstract
Introduction
Classical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cells have been widely used for the propagation of CSFV, but this cell line is not efficient or homogeneously susceptible to viral infection.
Material and Methods
To achieve a homogeneous PK15 cell line which enabled high titre replication of CSFV, we used the limiting dilution cell cloning method.
Results
We developed two cell clones, PK15-1A6 and PK15-3B1, which respectively have high- and low-permissive phenotypes to CSFV infection. The PK15-1A6, PK15-3B1, and PK15 parent cells showed different characteristics in cell proliferation rate, susceptibility to CSFV infection, and CSFV production. The mean virus titres per millilitre reflected by TCID50 values in PK15-1A6, PK15-3B1, and PK15 parent cells were 106.85, 103.63, and 104.74, respectively.
Conclusion
The PK15-1A6 cell clone is more permissive to CSFV infection than the PK15 parent cells. The screened high-permissive cells will be useful for CSFV propagation and vaccine development in vitro, and facilitate research on the pathogenicity of CSFV.
Publisher
Walter de Gruyter GmbH
Subject
General Veterinary
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