Inter-laboratory validation of liquid chromatography–tandem mass spectrometry multi-mycotoxin determination in animal feed – method transfer from the reference laboratory to regional laboratories-Reference-Cited by-同舟云学术

Inter-laboratory validation of liquid chromatography–tandem mass spectrometry multi-mycotoxin determination in animal feed – method transfer from the reference laboratory to regional laboratories

Published:2023-09-01 Issue:3 Volume:67 Page:397-406
ISSN:2450-8608
Container-title:Journal of Veterinary Research
language:en
Short-container-title:

Author:

Jedziniak Piotr¹,Panasiuk Łukasz¹,Pietruszka Katarzyna¹,Kolano Krzysztof²,Zbytniewski Radosław³

Affiliation:

1. 1 Department of Pharmacology and Toxicology, National Veterinary Research Institute , Puławy , Poland

2. 2 Department of Veterinary Hygiene, Provincial Veterinary Inspectorate , Kraków , Poland

3. 3 Department of Veterinary Hygiene, Provincial Veterinary Inspectorate , Bydgoszcz , Poland

Abstract

Abstract Introduction The results are presented of the inter-laboratory validation of a liquid chromatography–tandem mass spectrometry method for the determination of eight mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1, fumonisin B2, ochratoxin A, toxin T-2, toxin HT-2 and zearalenone) in animal feeds. Material and Methods This study was an essential part of the method’s transfer from the National Reference Laboratory to six regional laboratories in Poland working in the official survey of mycotoxins in feed. The laboratories received a batch of standard solutions, blank samples and quality control materials on which to perform analysis with one procedure and different liquid chromatography–tandem mass spectrometry conditions. Results The validation results show good precision (reproducibility coefficient of variation 3.7–20.5%) and accuracy of the method (recovery 89–120% and trueness 94–103%) and sufficient skills of the laboratory personnel. Conclusion The study is an example of the successful transfer of the method among laboratories.

Publisher

Walter de Gruyter GmbH

Subject

General Veterinary

Link

https://www.sciendo.com/pdf/10.2478/jvetres-2023-0045

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