Tk-deleted pseudorabies virus retains high pathogenicity in rats-Reference-Cited by-同舟云学术

Tk-deleted pseudorabies virus retains high pathogenicity in rats

Published:2021-10-26 Issue:4 Volume:65 Page:401-405
ISSN:2450-8608
Container-title:Journal of Veterinary Research
language:en
Short-container-title:

Author:

Zhang Lirong¹,Ruan Keyue¹,Sang Guoju¹,Xu Zhaoyang¹,Tong Wu¹,Yu Hai¹,Shan Tongling¹,Gao Fei¹,Li Liwei¹,Kong Ning¹,Tong Guangzhi¹²,Zheng Hao¹²

Affiliation:

1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Shanghai 200241 , China

2. Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University , Yangzhou 225009 , China

Abstract

Abstract Introduction The pseudorabies virus (PRV) gene encoding thymidine kinase (tk) is an important virulence-associated factor. Attenuation of PRV in susceptible animals is a frequent result of tk deletion. The aim of the study was to assess the pathogenicity of tk-deleted PRV in rats. Material and Methods Sprague Dawley rats were infected with the tk-deleted PRV strain SuHV-1 ΔTK:247via intranasal or intramuscular inoculation. PRV loads in ten tissues from dead and euthanised rats were determined using real-time PCR. Results Infection with SuHV-1 ΔTK:247 could cause death in rats. The 50% lethal dose (LD50) of SuHV-1 ΔTK:247 via intranasal inoculation was 103.16 TCID50 in rats. Intramuscular inoculation required a higher dose of SuHV-1 ΔTK:247 (105.0 TCID50). A high SuHV-1 ΔTK:247 titre was observed in the trigeminal ganglia or spinal cord of dead rats. Conclusion The results of this study show that rats are highly susceptible to PRV infection, and tk deletion did not completely diminish the pathogenicity of PRV in rats.

Publisher

Walter de Gruyter GmbH

Subject

General Veterinary

Link

https://www.sciendo.com/pdf/10.2478/jvetres-2021-0056

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