Study on the expression of testin in the testes of dogs-Reference-Cited by-同舟云学术

Study on the expression of testin in the testes of dogs

Published:2023-12-01 Issue:4 Volume:67 Page:627-633
ISSN:2450-8608
Container-title:Journal of Veterinary Research
language:en
Short-container-title:

Author:

Ciaputa Rafał¹^ORCID,Nowak Marcin¹^ORCID,Dzimira Stanisław¹^ORCID,Brambilla Eleonora²^ORCID,Kandefer-Gola Małgorzata¹^ORCID,Tomaszek Alicja¹^ORCID,Popiel-Kopaczyk Aneta³^ORCID,Dzięgiel Piotr³^ORCID,Grieco Valeria²^ORCID

Affiliation:

1. 1 Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences , Wrocław , Poland

2. 2 Department of Veterinary Medicine and Animal Science, University of Milan , Lodi , Italy

3. 3 Division of Histology and Embryology, Department of Human Morphology and Embryology, Wrocław Medical University , Wrocław , Poland

Abstract

Abstract Introduction Testin is a protein involved in cell mobility, adhesion and colony formation. In rats, testin presence has been reported in the testes, and its possible role in spermatogenesis has been suggested. Studies in humans also suggest a possible role of testin as a cancer suppressor protein. In the dog, which represents both an important pet species and a good animal model for studying biological and pathological testicular processes, the presence of testin has never been reported. Material and Methods In the present study, the expression of testin in foetal, prepubertal, adult and aged canine testes was investigated. Testes from 5 adult and 3 aged dogs, from 2 one-month-old puppies and from 2 foetuses miscarried at the end of pregnancy were immunohistochemically examined with a commercial antibody against testin. Results Testin was intensely expressed in Sertoli cells in every testis examined. Spermatids were also positive for testin in mature dogs and in the testicular areas of the aged ones which were not atrophic. Weak expression of testin was also detected in all testes examined. Conclusion The present study, the first demonstrating the presence of testin in canine testes, provides the basis for further dog–human comparative research and for studies on the role of this protein in canine physiology, reproduction and testicular pathologies.

Publisher

Walter de Gruyter GmbH

Subject

General Veterinary

Link

https://www.sciendo.com/pdf/10.2478/jvetres-2023-0055

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