Complete genome sequences of three sub-genotype 2.1b isolates of classical swine fever virus in China
Author:
Liu Chunxiao12, Li Mingliang1, Yin Xingwang1, Zhang Hongliang2, Xiang Lirun2, Zhai Hongyue1, Wang Congcong1, Kan Yunchao1, Yao Lunguang1, Tian Zhijun2, Leng Chaoliang1
Affiliation:
1. Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Laboratory of Insect Biology , Nanyang Normal University , Nanyang , 473061 , China 2. State Key Laboratory of Veterinary Biotechnology , Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin , 150069 , China
Abstract
Abstract
Introduction
Classical swine fever (CSF) has caused severe economic losses in pig production in many countries. Recent CSF outbreaks in China are mainly associated with sub-genotype 2.1 of CSF virus (CSFV). Although there is abundant information regarding 2.1 isolates, few data are available on whole-genome analysis.
Material and Methods
The biological and genome characteristics of three recently emerged Chinese CSFV isolates, i.e. SD2014-1, SD2014-2, and SD2014-3, were fully analysed.
Results
Sequence analysis showed that the isolates shared 83.4%–95.0% nucleotide identity with eight other CSFV isolates. In addition, the 5′ untranslated region (5′UTR) and the non-structural (NS) proteins NS3, NS4A, and NS4B were more conserved than other regions of the genome. Phylogenetic analysis based on the complete genome sequences or full-length structural protein E2 gene sequences revealed that the three isolates belonged to sub-genotype 2.1b. In addition, several unique molecular characteristics of the 5′UTR, 3′UTR, and E2 were identified.
Conclusion
The genomic variations of the three isolates will support further analysis of virulence determinants and the evolutionary trend of CSFV.
Publisher
Walter de Gruyter GmbH
Subject
General Veterinary
Reference31 articles.
1. Bjorklund H.V., Stadejek T., Vilcek S., Belak S.: Molecular characterization of the 3′ noncoding region of classical swine fever virus vaccine strains. Virus Genes 1998, 16, 307–312. 2. Chen Y., Xiao J., Xiao J., Sheng C., Wang J., Jia L., Zhi Y., Li G., Chen J., Xiao M.: Classical swine fever virus NS5A regulates viral RNA replication through binding to NS5B and 3′UTR. Virology 2012, 432, 376–388. 3. Coronado L., Liniger M., Munoz-Gonzalez S., Postel A., Perez L.J., Perez-Simo M., Perera C.L., Frias-Lepoureau M.T., Rosell R., Grundhoff A., Indenbirken D., Alawi M., Fischer N., Becher P., Ruggli N., Ganges L.: Novel poly-uridine insertion in the 3′UTR and E2 amino acid substitutions in a low virulent classical swine fever virus. Vet Microbiol 2017, 201, 103–112. 4. Deng M.C., Huang C.C., Huang T.S., Chang C.Y., Lin Y.J., Chien M.S., Jong M.H.: Phylogenetic analysis of classical swine fever virus isolated from Taiwan. Vet Microbiol 2005, 106, 187–193. 5. Edwards S., Fukusho A., Lefevre P.C., Lipowski A., Pejsak Z., Roehe P., Westergaard J.: Classical swine fever: the global situation. Vet Microbiol 2000, 73, 103–119.
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