Effect of β-hydroxybutyrate acid on gene expression levels of antioxidant biomarkers and growth hormone–related genes in liver cell culture-Reference-Cited by-同舟云学术

Effect of β-hydroxybutyrate acid on gene expression levels of antioxidant biomarkers and growth hormone–related genes in liver cell culture

Published:2024-06-01 Issue:2 Volume:68 Page:313-324
ISSN:2450-8608
Container-title:Journal of Veterinary Research
language:en
Short-container-title:

Author:

Mohsin Muhammad Ali¹^ORCID,Zhou Xiaojing¹^ORCID,Huiru Yu¹²^ORCID,Shen Wenxiang¹^ORCID,He Baoxiang¹^ORCID,Sobiech Przemysław³^ORCID,Pierzchała Mariusz⁴^ORCID,Ogłuszka Magdalena⁴,Starzyński Rafał⁴,Kalra Garima⁵^ORCID,Deshmukh Bharti⁵^ORCID,Thangarasu Revathy⁶,Kashyap Neeraj⁶,Czarnik Urszula⁷,Lepczyński Adam⁸,Woźniakowski Grzegorz⁹¹⁰,Pareek Chandra S.⁹¹⁰^ORCID

Affiliation:

1. Department of Clinical Veterinary Medicine, College of Animal Science and Technology, Guangxi University , Nanning , , Guangxi , China

2. Shanghai Animal Disease Prevention and Control Center , Changning District , , Shanghai , China

3. Department of Clinical Sciences, Internal Disease Unit, Faculty of Veterinary Medicine , Utrecht , Netherlands

4. Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences , Magdalenka , Poland

5. Department of Animal Genetics and Breeding , Ludhiana , Punjab , India

6. College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University , Ludhiana , , Punjab , India

7. Department of Pig Breeding, Faculty of Animal Bio-Engineering, University of Warmia and Mazury in Olsztyn , Olsztyn , Poland

8. Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology , Szczecin , Poland

9. Department of Infectious and Invasive Diseases and Veterinary Administration, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University , Toruń , Poland

10. Division of Functional Genomics in Biological and Biomedical Research, Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University , Toruń , Poland

Abstract

Abstract Introduction In dairy cattle, oxidative stress is a predominant problem associated with diseases and reproductive health issues. This study aimed to detect the variation in the antioxidant biomarkers by adding different concentrations of β-hydroxybutyric acid (BHBA) and sought to elucidate its effects on the gene expression levels of growth hormone (GH) and antioxidant biomarkers in bovine hepatocytes. Material and Methods Four antioxidant biomarkers, namely malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH Px) were evaluated using commercially available bovine ELISA kits. The expression levels of the bovine GH, its receptor (GHR), insulin-like growth factor (IGF), IGF-1, IGF-1 receptor, CAT, SOD, GSH-Px and β-actin (as a reference) genes in liver cell culture were determined by reverse transcriptase-PCR assay. Results With the increase of BHBA concentration and culture time, the activities of SOD, CAT, and GSH Px biomarkers in hepatocytes decreased. However, the content of MDA in hepatocytes increased gradually with the increase of hepatocyte culture time and BHBA concentration. The qPCR results revealed that after adding BHBA, gene expression levels of GSH-Px, SOD and IGF biomarkers in hepatocytes began to differ in the culture groups at 12 h, whereas the gene expression level of the CAT and GHR biomarkers in hepatocytes began to differ at 6 h. Conclusion Quantitative PCR results showed that the BHBA significantly downregulated the expression levels of the GHR gene and CAT, GSH Px and SOD antioxidant biomarker genes.

Publisher

Walter de Gruyter GmbH

Link

https://www.sciendo.com/pdf/10.2478/jvetres-2024-0037

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