The role of leukotriene B<sub>4</sub> in cow metritis-Reference-Cited by-同舟云学术

The role of leukotriene B₄ in cow metritis

Published:2023-03-01 Issue:1 Volume:67 Page:99-104
ISSN:2450-8608
Container-title:Journal of Veterinary Research
language:en
Short-container-title:

Author:

Wang Guo-Qing¹,Zheng Hao-Yue¹,Hou Jin-Long¹,Wang Cui¹,Sun Hui-Lian¹,Wang Lan¹

Affiliation:

1. College of Life and Health Sciences, Northeastern University , Shengyang 110819 , P.R. China

Abstract

Abstract Introduction Metritis is a common postpartum disease in dairy cows. As a mast cell (MC) mediator, leukotriene B4 (LTB4) is the strongest phagocyte chemokine. It is important in inflammation for the recruitment of immune cells to resist infection. This study investigated the effect of LTB4 in metritis. Material and Methods Twenty Holstein cows 3 to 6 years old and at 6 to 10 days postpartum were selected, ten of which with postpartum metritis were the experimental group, and the other ten of which as healthy cows were the control group. The levels of LTB4, substance P (SP) and vasoactive intestinal peptide (VIP) were measured by ELISA, the expression of LTB4 receptor 2 (BLT2), matrix metalloproteinase (MMP)-2 and MMP-9 mRNA was measured by qPCR, and collagens I and IV were detected by immunohistochemical staining. Results Concentrations of SP and LTB4 were significantly higher, but those of VIP were significantly lower in the experimental group than those in the control group. The expression of BLT2, MMP-2 and MMP-9 mRNA was significantly higher in the experimental group than that in the control group. The expression of collagen Ⅰ and collagen Ⅳ was significantly lower in the experimental group than that in the control group. Conclusion In metritis, SP promotes the activation of MC and the synthesis and release of LTB4. Leukotriene B4 chemotactic immune cells promote the high expression of collagenase, which accelerated the hydrolysis of collagen, while the inhibitory effect of VIP on MC was weakened. This may further aggravate the damage to uterine tissue.

Publisher

Walter de Gruyter GmbH

Subject

General Veterinary

Link

https://www.sciendo.com/pdf/10.2478/jvetres-2023-0011

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