The Genetic Analysis of Potential New HEV-3 Subtype Identified on Pig Farm in Slovakia
Author:
Kočíková Božena1, Pavlová Alica1, Dudášová Katarína1, Mandelík René1, Jacková Anna1
Affiliation:
1. 1 Department of Epizootiology, Parasitology and Protection of One Health , University of Veterinary Medicine and Pharmacy in Košice , Komenského 73, 041 81 Košice , Slovakia
Abstract
Abstract
Hepatitis E virus (HEV) represents the causative agent of acute viral hepatitis in the world. The virus belongs to the genus Paslahepevirus, species Paslahepevirus balayani, which contains 8 different genotypes and at least 14 reference HEV-3 subtypes. Recently we have identified three isolates in a Slovakian pig farm as a new unclassified HEV-3 subtype. The aim of this study was to sequence and analyze additional regions of HEV genome for PER5 isolate, a representative isolate of potential new HEV-3 subtype. The phylogenetic analyses of 749 bp and 828 bp DNA fragments amplified from ORF1 revealed that both sequences fall outside two major clades (3abchij and 3efg) of the HEV-3 genotype as well as the other HEV-3 subtypes. The nucleotide identity with other HEV-3 subtypes indicated low values within the range 79.2–84.1 % as well. The results provide further support for the circulation of a new HEV-3 subtype in the pig farm in Slovakia.
Publisher
Walter de Gruyter GmbH
Reference31 articles.
1. 1. Adlhoch, C., Wolf, A., Meisel, H., Kaiser, M., Ellerbrok, H., Pauli, G., 2009: High HEV presence in four different wild boar populations in East and West Germany. Vet. Microbiol., 139, 3–4, 270–278. DOI: 10.1016/j.vetmic.2009.06.032. 2. 2. Bigoraj, E., Paszkiewicz, W., Rzeżutka, A., 2021: Porcine blood and liver as sporadic sources of hepatitis E virus (HEV) in the production chain of offal-derived foodstuffs in Poland. Food Environ. Virol., 13, 3, 347–356. DOI: 10.1007/ s12560-021-09475-z. 3. 3. Capai, L., Charrel, R., Falchi, A., 2018: Hepatitis E in high-income countries: What do we know? And what are the knowledge gaps ? Viruses, 10, 6, 285. DOI: 10.3390/v10060285. 4. 4. Colson, P., Borentain, P., Queyriaux, B., Kaba, M., Moal., V., Gallian, P., et al., 2010: Pig liver sausage as a source of hepatitis E virus transmission to humans. J. Infect. Dis., 202, 6, 825–834. DOI: 10.1086/655898. 5. 5. De Sabato, L., Lemey, P., Vrancken, B., Bonfanti, L., Ceglie, L., Vaccari, G., et al., 2018: Proposal for a new subtype of the zoonotic genotype 3 hepatitis E virus: HEV-3l. Virus Research, 248, 1–4. DOI: 10.1016/j.virusres.2018.02.010.
|
|