DOPE–oleic acid–Ca2+ as DNA condensing agent

Author:

Galliková D.,Liskayová G.,Búcsi A.,Hubčík L.,Martínez J.C.,Uhríková D.

Abstract

Abstract Phospholipid-based non-viral carriers composed of neutral phospholipid dioleoylphosphatidylethanolamine (DOPE) and the binary mixture DOPE–oleic acid (OA) are examined as potential DNA delivery vectors. The process of DNA condensation in the presence of Ca2+ ions has been monitored through changes in emmision intensity of fluorescent probe ethidium bromide. The decline in fluorescence intensity with increasing Ca2+ concentration at two different time intervals was correlated with the binding capacity of complexes and possible release of DNA from the complex. The microstructure of DOPE–OA mixtures at different OA/DOPE molar ratios and that of DOPE–OA–DNA–Ca2+ complexes were determined using synchrotron small angle X-ray diffraction (SAXD). We identified inverted hexagonal phase HII as the dominant structure. OA affects the lattice parameter of HII formed by DOPE. With the increasing OA/DOPE molar ratio, the lattice parameter decreases, which results in significantly lower fraction of DNA bound to the OA-enriched complexes.

Publisher

Walter de Gruyter GmbH

Subject

General Pharmacology, Toxicology and Pharmaceutics

Reference32 articles.

1. Stimuli Responsive Polymorphism of NO DOPE DNA Complexes : Effect of PH , Temperature and Composition Biochimica - https org;Hubčík;et Biophysica Acta Biomembranes,2015

2. Oleic Acid - Induced Mitogenic Signaling in Vascular Smooth Muscle Cells A Role for Protein Kinase;Lu;Circulation Research,1996

3. Ionization and Phase Behavior of Fatty Acids in Water : Application of the Gibbs Phase Rule;Cistola;Biochemistry,1988

4. The Structure of DNA DOPC Aggregates Formed in Presence of Calcium and Magnesium Ions - Angle Synchrotron Ray Diffraction Study Biochimica et - https org bbamem;Uhríková;Small Biophysica Acta Biomembranes,2005

5. Effects of Unsaturated Fatty Acids and Triacylglycerols on Phosphatidylethanolamine Membrane Structure of Lipid https org;Prades;Journal Research,2003

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3