Heterologous expression of Bacillus subtilis SL-44 glycosyltransferase catalyzed synthesis of ginsenoside Rh2
Author:
Zhang Yufei1, Liu Xiaochen12, Li Zhiyan1, Yue Junsong1, Pan Hong12
Affiliation:
1. 1 School of Environmental and Chemical Engineering, Xi’an Laboratory of Textile Chemical Engineering Auxiliaries , Xi’an Polytechnic University , Xi’an , Shaanxi , , China . 2. 2 Shaanxi Key Laboratory of Degradable Biomedical Materials , School of Chemical Engineering, Northwest University , Xi’an , Shaanxi , , China .
Abstract
Abstract
In this paper, the synthesis of ginsenoside Rh2 was catalyzed by using heterologous expression of Bacillus subtilis SL-44 glycosyltransferase. The synthesis parameters of ginsenoside Rh2 were optimized by the selection of strains and chemical supplies, the establishment of kinetic equations for the respiration rate of UGT enzyme, the effect of storage temperature on the model, and the glycosylation reaction of ginsenoside PPD with UGT. The effect of Rh2 saturation on the thermal denaturation temperature of the protein was analyzed along with the kinetic properties of the enzyme GE02773 (GE03484) while varying the saturation of Rh2. The results showed that the conversion of ginsenoside Rh2 reached 84% at a temperature of 35℃, pH 8, 5% DMSO, 0.4 of M-UDPG, and 1M-PPD in reaction with GE02773. In this paper, we successfully achieved the efficient synthesis of ginsenoside Rh2, which provides new ways and ideas for the application and synthesis of ginsenoside Rh2, with important practical significance and scientific value.
Publisher
Walter de Gruyter GmbH
Subject
Applied Mathematics,Engineering (miscellaneous),Modeling and Simulation,General Computer Science
Reference24 articles.
1. Park, J. E., Ji, H. W., Kim, H. W., Baek, M., Jung, S., & Sun, J. K. (2022). Ginsenoside rh2 regulates the cfap20dc-as1/microrna-3614-3p/bbx and tnfaip3 axis to induce apoptosis in breast cancer cells. The American journal of Chinese medicine, 50(6), 1703-1717. 2. Chu, J., Yue, J., Qin, S., Li, Y., & He, B. (2021). Biocatalysis for rare ginsenoside rh2 production in high level with co-immobilized udp-glycosyltransferase bs-yjic mutant and sucrose synthase atsusy. Catalysts, 11(1), 132. 3. Jin, X., Yang, Q., Cai, N., & Zhang, Z. (2020). A cocktail of betulinic acid, parthenolide, honokiol and ginsenoside rh2 in liposome systems for lung cancer treatment. Nanomedicine, 15(1), 41-54. 4. Gao, Qiruo, Zheng, & Junhua. (2018). Ginsenoside rh2 inhibits prostate cancer cell growth through suppression of micro rna rna ‐4295 that activates cdkn cdkn 1a. Cell Proliferation, 51(3), art. no. e12438-art. no. e12438. 5. Yan, H., Jin, H., Fu, Y., Yin, Z., & Yin, C. (2019). Production of rare ginsenosides Rg3 and Rh2 by endophytic bacteria from Panax ginseng. Journal of agricultural and food chemistry, 67(31), 8493-8499.
|
|