Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods-Reference-Cited by-同舟云学术

Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

Published:2014-06-01 Issue:2 Volume:58 Page:219-222
ISSN:2300-3235
Container-title:Bulletin of the Veterinary Institute in Pulawy
language:en
Short-container-title:

Author:

Nadăş George Cosmin¹,Kalmár Zsuzsa²,Taulescu Marian Aurel³,Chirilă Flore¹,Bouari Cosmina Maria³,Răpuntean Sorin¹,Bolfa Pompei³⁴,Fiţ Nicodim Iosif¹

Affiliation:

1. Department of Microbiology-Immunology

2. Department of Parasitology and Parasitic Diseases

3. Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania

4. Department of Biomedical Sciences, Ross University School of Veterinary Medicine Basseterre, P.O. Box 334, Basseterre, St. Kitts, West Indies

Abstract

Abstract The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.

Publisher

Walter de Gruyter GmbH

Link

https://www.sciendo.com/pdf/10.2478/bvip-2014-0032

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