Simultaneously both expression of LMP-1 and methylation of E-cadherin: Molecular biomarker in stage IV of nasopharyngeal carcinoma patients

Author:

Lao TD1,Truong PK1,Thieu HH1,Nguyen DH2,Nguyen MT3,Le TAH1

Affiliation:

1. Department of Pharmaceutical and Medical Biology, Ho Chi Minh City Open University , Ho Chi Minh City , Vietnam

2. Ear, Nose and Throat Department, University of Medicine and Pharmacy at Ho Chi Minh City , Ho Chi Minh City , Vietnam

3. Department of Otorhinolaryngology, Cho Ray Hospital , Ho Chi Minh City , Vietnam

Abstract

Abstract The phenome of E-cadherin gene methylation and the expression of latent membrane protein 1 (LMP-1) gene are associated with nasopharyngeal carcinoma (NPC). In order to determine whether cooperative LMP-1 expression or methylation of E-cadherin could serve as the potential molecule biomarker target for diagnosis and therapy of NPC, a case-control study including 93 NPC biopsy samples and 100 non cancerous nasopharyngeal swab samples were examined, as well as the strength of association among them by the quantitative polymerase chain reaction (qPCR) and nested-methylation-specific PCR methods. The significantly higher frequency of LMP-1 expression and E-cadherin methylation in NPC biopsy samples, accounting for 76.34 and 73.12%, respectively, compared to non cancerous samples, accounting for 0.00 and 30.00%, respectively, were observed. The significant correlation between the LMP-1 expression and E-cadherin methylation in NPC samples was reported. In detail, in the stage IV of NPC, in case of LMP-1-positive samples, 35 of 37 samples (accounting for 94.60%) were positive for methylation of E-cadherin. It was demonstrated that cooperative LMP-1 expression and E-cadherin gene methylation could serve as a molecular biomarker in NPC.

Publisher

Walter de Gruyter GmbH

Subject

Genetics (clinical),Genetics

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