The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs-Reference-Cited by-同舟云学术

The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs

Published:2013-03-01 Issue:1 Volume:16 Page:131-133
ISSN:1505-1773
Container-title:Polish Journal of Veterinary Sciences
language:
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Author:

Adaszek Ł.,Jankowska M.,Kalinowski M.,Banach T.,Wułupek D.,Winiarczyk S.

Abstract

Abstract The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.

Publisher

Walter de Gruyter GmbH

Subject

General Veterinary,General Medicine

Link

https://www.degruyter.com/view/j/pjvs.2013.16.issue-1/pjvs-2013-0020/pjvs-2013-0020.pdf

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