The Performance of Seven Molecular Methods for the Detection of PRRSV
Author:
Milićević Vesna1, Kureljušić Branislav1, Veljović Ljubiša1, Valčić Miroslav2, Stević Nataša2, Savić Božidar1, Radojičić Sonja2
Affiliation:
1. Institute of Veterinary Medicine of Serbia , Janisa Janulisa 14, Belgrade , Serbia 2. Faculty of Veterinary Medicine , University in Belgrade , Bulevar Oslobođenja 18, Belgrade , Serbia
Abstract
Abstract
Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.
Publisher
Walter de Gruyter GmbH
Subject
General Veterinary
Reference20 articles.
1. 1. Dewey C: PRRS in North America, Latin America, and Asia. Vet. Res. 2000, 84-85.10.1051/vetres:2000015 2. 2. Novosel D, Petrović T, Acinger-Rogić Ž, Štukelj M: Epidemiology and status of Porcine Reproductive and Respiratory Syndrome in the Western Balkan region: challenges and prospects. Slov Vet Res 2016, 53: 185-93. 3. 3. Cavanaugh D: Nidovirales a new order comprising Corona-viridae and Arteriviridae. Arch Virol 1997, 142: 629-633. 4. 4. Nelson EA, Christopher-Hennings J, Drew T, Wensvoort G, Collins JE, Benfield DA: Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies. J Clin Microbiol 1993, 31: 3184–3189.10.1128/jcm.31.12.3184-3189.1993 5. 5. Tian K, Yu X, Zhao T, Feng Y, Cao Z, Wang C, Hu Y, Chen X, Hu D, Tian X, Liu D, Zhang S, Deng X, Ding Y, Yang L, Zhang Y, Xiao H, Qiao M, Wang B, Hou L, Wang X, Yang X, Kang L, Sun M, Jin P, Wang S, Kitamura Y, Yan J, Gao GF: Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS One 2007, 2:e526.10.1371/journal.pone.0000526
|
|